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Fig. 1. MPs promote in vitro tube formation of Eahy 926 endothelial cells. Phase-contrast micrographs showing that 10 lg/ml MPs carrying Shh induce network formation on Matrigel in Eahy 926 <t>cells.</t> <t>Silencing</t> Shh pathway by either the pharmacological inhibitor cyclopamine (30 lM) or <t>siRNA</t> of the Shh receptor, Patched-1, prevented MP-induced capillary formation. However, siRNA scrambled had no effect on in vitro angiogenesis evoked by MPs. Reproducible data were obtained from four independent experiments.
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Fig. 1. MPs promote in vitro tube formation of Eahy 926 endothelial cells. Phase-contrast micrographs showing that 10 lg/ml MPs carrying Shh induce network formation on Matrigel in Eahy 926 cells. Silencing Shh pathway by either the pharmacological inhibitor cyclopamine (30 lM) or siRNA of the Shh receptor, Patched-1, prevented MP-induced capillary formation. However, siRNA scrambled had no effect on in vitro angiogenesis evoked by MPs. Reproducible data were obtained from four independent experiments.

Journal: Carcinogenesis

Article Title: Microparticles harboring Sonic Hedgehog promote angiogenesis through the upregulation of adhesion proteins and proangiogenic factors.

doi: 10.1093/carcin/bgp030

Figure Lengend Snippet: Fig. 1. MPs promote in vitro tube formation of Eahy 926 endothelial cells. Phase-contrast micrographs showing that 10 lg/ml MPs carrying Shh induce network formation on Matrigel in Eahy 926 cells. Silencing Shh pathway by either the pharmacological inhibitor cyclopamine (30 lM) or siRNA of the Shh receptor, Patched-1, prevented MP-induced capillary formation. However, siRNA scrambled had no effect on in vitro angiogenesis evoked by MPs. Reproducible data were obtained from four independent experiments.

Article Snippet: In order to silencing Patched-1, the Shh receptor, siRNA duplexes specific for human Patched-1 and control, non-silencing siRNA was obtained from Santa Cruz Biotechnology.

Techniques: In Vitro

Fig. 2. MPs enhance in vitro tube formation of HUVECs. (A) Phase-contrast micrographs showing that 10 lg/ml MPs carrying Shh favor network formation on Matrigel in HUVECs. Silencing Shh pathway with the pharmacological inhibitor cyclopamine (Cycl, 30 lM) or siRNA of the Shh receptor, Patched-1, prevented MP-induced capillary formation. Conditioned media obtained from HUVECs treated with MPs were unable to induce capillary formation. VEGF (20 ng/ml) was used as positive control. (B) Capillary length was used to quantify angiogenesis. Reproducible data were obtained from three independent experiments. P , 0.05 versus in the absence of MPsShhþ. #P , 0.05 versus in the presence of MPsShhþ alone or VEGF alone.

Journal: Carcinogenesis

Article Title: Microparticles harboring Sonic Hedgehog promote angiogenesis through the upregulation of adhesion proteins and proangiogenic factors.

doi: 10.1093/carcin/bgp030

Figure Lengend Snippet: Fig. 2. MPs enhance in vitro tube formation of HUVECs. (A) Phase-contrast micrographs showing that 10 lg/ml MPs carrying Shh favor network formation on Matrigel in HUVECs. Silencing Shh pathway with the pharmacological inhibitor cyclopamine (Cycl, 30 lM) or siRNA of the Shh receptor, Patched-1, prevented MP-induced capillary formation. Conditioned media obtained from HUVECs treated with MPs were unable to induce capillary formation. VEGF (20 ng/ml) was used as positive control. (B) Capillary length was used to quantify angiogenesis. Reproducible data were obtained from three independent experiments. P , 0.05 versus in the absence of MPsShhþ. #P , 0.05 versus in the presence of MPsShhþ alone or VEGF alone.

Article Snippet: In order to silencing Patched-1, the Shh receptor, siRNA duplexes specific for human Patched-1 and control, non-silencing siRNA was obtained from Santa Cruz Biotechnology.

Techniques: In Vitro, Positive Control